Rodent training

 

 

 

Registration Process

  1. Complete the online registration form to reserve your preferred lab training date and time. To prevent delays, please ensure that the form is complete.
    Note: You will receive a copy of the registration form by email within 10 minutes of submitting. If you do not receive a copy of the registration form, contact the Training Coordinator.
  2. A confirmation email will be sent to your Primary Investigator (PI) for approval.
  3. The Training Coordinator will email you the following information:
    • Your confirmed training date(s)
    • Details on your registered lab training session
    • Login information for the required online tutorial(s) (see below)

Please allow the Training Coordinator three working days to coordinate your registration. If you have not received this information within three working days, please send a request to the Training Coordinator.

  • All prerequisites must be completed no later than 1pm the day prior to your reserved date of training.
  • Lab registration deadline is 5 working days prior to the available wet lab.
  • *Neuro3605 students are required to complete the registration form for Module 3: Rodent Research Facility Orientation Lab*

Returning after not handling animals

If you are a previous animal user returning to animal use after an extended period of not handling animals (typically 6 months or longer), you will be required to repeat the Orientation Online Tutorial (Module 3), the Handling Online Tutorial (Module 4) and be assessed for animal handling proficiency.

 

Registration examples

Lisa is a 3rd year undergrad that will be working in Lab X for the summer. She will be working with rats and will be helping a grad student by assisting in surgeries. The courses that Lisa will need to register for are:

  • Module 5: Rat Handling Lab
  • Module 7: Aseptic Technique Lab
  • Module 9: Rodent Anaesthesia Lab

After successful registration, the Training Coordinator will contact Lisa with all of the online tutorial prerequisites that are required to complete 24 hours prior to attending the scheduled lab.

Bob is a grad student that will be working in Lab X for his Master's study. He will be working with mice and will be performing surgeries as part of his study. The courses that Bob will need to register for are:

  • Module 5: Mouse Handling Lab
  • Module 7: Aseptic Technique Lab
  • Module 9: Rodent Anaesthesia Lab
  • Module 11: Rodent Basic Surgery Course
  • Module 12: Competency Review

After successful registration, the Training Coordinator will contact Bob with all of the online tutorial prerequisites that are required to complete 24 hours prior to attending the scheduled lab.

Tom is a laser technician that is working with Lab X. He will not be handling animals or will need to go into any of the animal housing rooms. He will only need to access the Rodent Research Facility to work on the laser equipment. The courses that Tom will need to register for are:

  • Module 3: Rodent Research Facility Orientation Lab

After successful registration, the Training Coordinator will contact Tom with all of the online tutorial prerequisites that are required to complete 24 hours prior to attending the scheduled lab.

Mandatory modules

Online tutorial

Information on how to prevent workplace injuries resulting from storage and use of hazardous products.

  • Prerequisites: None
  • Quiz: 70% to pass
  • Allow 2 hours to complete

Online tutorial (also known as Part 1)

Ethics, guidelines, regulations and safety information.

  • Prerequisites: PI approval
  • Quiz: 72% to pass
  • Allow 4 - 6 hours to complete.

Online tutorial

Facility regulation, personal protective equipment (PPE), decontamination process, working alone, campus accident/incident reporting and submission of a 1/2 page essay on your personal view on the use of animals in research.

Note: Individuals that require access to the Rodent Research Facility and will not be working with animals, need to register for the Orientation Lab on the registration form.

  • Prerequisites: PI approval
  • Quiz: 70% to pass
  • Allow 2 hours to complete

Rat handling

Upon successful completion of Module 5, you will receive the Rat Handling Certification.

3R's, history, biology, breeding, husbandry, common health concerns & diseases, sex determination, identification, recognizing pain and distress, endpoints, handling, restraint, safety concerns, cage cards and colony management program.

  • Prerequisites: PI approval, Modules 1-3
  • Quiz: 70% to pass
  • Allow 4 hours to complete

This lab will allow you to familiarize yourself with the species to gain confidence in handling them. Topics discussed will include facility orientation and regulations, humane handling, common methods for identifying animals, sex determination and performing a health assessment.

You will be required to show competency in sex determination, health assessment and humane handling of the rat prior to receiving certification.

  • Prerequisites: PI approval, Modules 1-4

Mouse handling

Upon successful completion of Module 5, you will receive the Mouse Handling Certification.

3R's, history, biology, breeding, husbandry, common health concerns & disease, sex determination, identification, recognizing pain and distress, endpoints, handling, restraint, safety concerns, cage cards and colony management program.

  • Prerequisites: PI approval, Modules 1-3
  • Quiz: 70% to pass
  • Allow 4 hours to complete

This lab will allow you to familiarize yourself with the species and gain confidence in handling them. Topics discussed will include facility orientation and regulations, common methods for identifying animals, humane handling & restraint, sex determination and performing a health assessment.

You will be required to show competency in sex determination, health assessment and humane handling & restraint of the mouse prior to receiving certification.

  • Prerequisites: PI approval, Modules 1-4

Aseptic technique

The purpose of this course is to prepare you with the skills and knowledge to assist a certified surgeon during surgery. Upon successful completion of Module 7, you will receive Aseptic Technique Course Completion Certification.

Instrument care, preparing surgical packs, autoclave use, preparing the surgery suite, recording and documentation, asepsis and biomethodology.

  • Prerequisites: PI approval, Modules 1-5
  • Quiz: 70% to pass
  • Allow 4 hours to complete

This lab will review all of the topics covered in Module 6 in a format that allows you to practice all techniques required for assisting in surgery.

  • Prerequisites: PI approval, Modules 1-6, a minimum of 1 month animal handling experience in the relevant species and can show proficiency in animal handling

Rodent anaesthesia

The purpose of this course is to prepare you with the skills and knowledge to assist a certified anesthetist. Upon successful completion of Module 9, you will receive the Anesthesia Course Completion Certification.

The certification does not include proof of competency, so you must be under direct supervision while inducing and maintaining anesthesia. If anesthesia competency certification is desired, a Competency Review can be requested by contacting the Training Coordinator.

Rodent anaesthetic, rodent analgesia, anaesthesia monitoring, anaesthesia recovery, recording and documentation.

  • Prerequisites: PI approval, Modules 1-5
  • Quiz: 70% to pass
  • Allow 4 hours to complete

This lab will cover all topics covered in the Module 8 in a format that allows you to practice all techniques required for assisting in surgery.

  • Prerequisites: PI approval, Modules 1-5, 8, a minimum of 1 month animal handling experience in the relevant species and can show proficiency in animal handling, Subcutaneous (SC) injection certification in the relevant species

Rodent basic surgery course

The purpose of this course is to prepare you with the skills and knowledge to complete anaesthesia and surgery unsupervised. Until certification is achieved, you are expected to work under direct supervision by a certified surgeon while performing surgery, anaesthesia and post-op care (where applicable). Upon successful completion of Modules 12, you will receive the Rodent Surgical Competency Certification.

Gowning and gloving, asepsis, wound healing, sterile tip procedure, sterotaxic basics, incision, skull preparation basics and suturing.

  • Prerequisites: PI approval, Modules 1-9
  • Allow 4 hours to complete

This lab will review all topics covered in Module 8 in a format that allows you to practice all techniques required for an introduction to surgery. You will be provided with a checklist of expectations to prepare for the competency review (Module 12).

  • Prerequisites: PI approval, Modules 1-10, a minimum of 1 month animal handling experience in the relevant species and can show proficiency in animal handling, Subcutaneous (SC) injection certification in the relevant species.

The purpose of the review is to ensure that you are competent to perform the anaesthesia, surgical procedure, and post-op care (where applicable) without supervision. The University Veterinarian will observe you through the entire anaesthetic, surgical procedure and post-op recovery.

Upon the University Veterinarian's approval, certification will be given to you. Certification is species specific and categorized as: Chronic Stereotax, Acute Stereotax or Other. Certification categories are not transferable to other categories or species.

  • Prerequisites: PI approval, Modules 1-11

Protocol-specific training

Upon successful completion of Module 14, you will receive certification in the protocol-specific technique for that species. The certification is not transferable to other rodent species.

Protocol specific training such as restraint, injections, blood collection, etc..

  • Prerequisites: PI approval, participation in a study that involves the requested technique, Modules 1-5
  • Quiz: 70% to pass

Protocol specific training such as restraint, injections, blood collection, etc.. Proficiency in handling will be required prior to attending the training lab.

You will be required to show competency in the procedure prior to receiving certification.

  • Prerequisites: PI approval, participation in a study that involves the requested technique, Modules 1-5, 13, can show proficiency in animal handling.
 
For more information on the training programs, contact the Training Coordinator.