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DNA fingerprinting of Alberta bull trout (Salvelinus confluentus) populations

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dc.contributor.advisor Thomas, James
dc.contributor.advisor Chew, G. Lincoln Groft, Donald G. University of Lethbridge. Faculty of Arts and Science 2007-04-20T21:16:52Z 2007-04-20T21:16:52Z 1997
dc.description xvii, 161 leaves : ill. ; 28 cm. en
dc.description.abstract Bull trout (Salvelinus confluentus) populations from Alberta river drainage systems were compared using molecular techniques. Restriction fragment length polymorphisms (RFLP's) within the NDI and ND5/6 regions of the mitochondrial genome were observed. In addition, randomly amplified polymorphic DNA profiles (RAPD's) from total genomic DNA extracts were compared. Interdrainage comparisons using mtDNA revealed significant population heterogeneity among Alberta bull trout. Percent sequence divergence in mtDNA ranged from 0.14% to 0.92%. Most fish in each population were composed of a small number of common haplotypes, and the remaining fish displayed rare or locally unique haplotypes. RAPD profiles were used to calculate genetic distance values for Alberta, Canada and Montana, U.S.A. populations. Both Nei and Cavalli-Sforza distance values were used to generate neighbor-joining, FITCH and KITSCH distance trees. Two genetically distinct groups of bull trout were revealed by the RAPD analysis and the possiblity that post-glacial bull trout populations are derived from two separate refugia is suggested. en
dc.language.iso en_US en
dc.publisher Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1997 en
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en
dc.subject Bull trout -- Alberta en
dc.subject DNA fingerprinting -- Alberta en
dc.subject Chars -- Alberta en
dc.subject Fishes -- Genetics en
dc.subject Fishes -- Alberta en
dc.subject Dissertations, Academic en
dc.title DNA fingerprinting of Alberta bull trout (Salvelinus confluentus) populations en
dc.type Thesis en
dc.publisher.faculty Arts and Science
dc.publisher.department Department of Biological Sciences Masters

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