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Effects of ammonium nitrate upon direct somatic embryogenesis and biolistic transformation of wheat

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dc.contributor.advisor Kovalchuk, Igor
dc.contributor.author Greer, Michael S.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2008-09-25T20:00:05Z
dc.date.available 2008-09-25T20:00:05Z
dc.date.issued 2008
dc.identifier.uri http://hdl.handle.net/10133/661
dc.description x, 81 leaves : ill. ; 29 cm. en
dc.description.abstract Triticum aestivum is of major importance both nutritionally and economically globally. Traditional breeding mechanisms have been unsuccessful at keeping pace with the increasing demand for better yielding and more resilient wheat varieties. The introduction of foreign genes into systems has provided a new tool for crop improvement, but has been difficult to apply to elite wheat varieties mainly as result of their recalcitrance to prerequisite tissue culture. Investigations here demonstrate that modification of the ammonium nitrate content in direct somatic embryogenesis induction medium can increase the number of primary embryos produced by over two fold in the elite hard red wheat cultivar Superb. The number of primary embryos which were capable of transitioning into shoot development also increased by two fold. Biolistic transformation efficiency was also improved when targeted scutellar tissue was exposed to elevated ammonium nitrate levels. en
dc.language.iso en_US en
dc.publisher Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2008 en
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en
dc.subject Dissertations, Academic en
dc.subject Electronic dissertations en
dc.subject Wheat -- Somatic embryogenesis en
dc.subject Ammonium nitrate en
dc.title Effects of ammonium nitrate upon direct somatic embryogenesis and biolistic transformation of wheat en
dc.type Thesis en
dc.publisher.faculty Arts and Science en
dc.publisher.department Department of Biological Sciences en

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