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Fusarium head blight of barley : resistance evaluation and identification of resistance mechanisms

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dc.contributor.advisor Eudes, François
dc.contributor.advisor Selinger, L. Brent Geddes, Jennifer M H University of Lethbridge. Faculty of Arts and Science 2007-07-24T21:03:47Z 2007-07-24T21:03:47Z 2006
dc.description xvii, 196 leaves : ill. ; 29 cm. en
dc.description.abstract An evaluation of nineteen barley lines using three artificial inoculation methods concluded that spray inoculation was the most reproducible method and provided the greatest discrimination of resistance. Six of the nineteen barley lines were used for proteomic studies to identify defense responses following F. graminearum infection. All lines responded by inducing an oxidative burst and pathogenesis-related proteins. Differences in response magnitude and the proteins activated could be attributed to varying levels of FHB resistance amongst the barley lines. RNA microarray profiling and iTRAQ technology were used to study the interaction between two barley lines under five different treatments testing the effect of the fungus, trichothecene, and their interaction. Resistance was differentiated by the early induction of defense-related genes and the activation of the JA and ethylene defense pathways in Chevron, compared to the induction of a less efficient defense pathway in Stander; observed intra- and inter-cultivar differential responses are discussed. en
dc.language.iso en_US en
dc.publisher Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2006 en
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en
dc.subject Dissertations, Academic en
dc.subject Barley -- Disease and pest resistance en
dc.subject Fusarium diseases of plants -- Prevention en
dc.title Fusarium head blight of barley : resistance evaluation and identification of resistance mechanisms en
dc.type Thesis en
dc.publisher.faculty Arts and Science en
dc.publisher.department Department of Biological Studies en

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