Plant organelle targeting cell penetrating peptides
Date
2014
Authors
MacMillan, Trevor
University of Lethbridge. Faculty of Arts and Science
Journal Title
Journal ISSN
Volume Title
Publisher
Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences
Abstract
To address the limitations of conventional nuclear transformation I have developed a peptide based gene delivery system to genetically manipulate the genomes of plant cell mitochondria and plastids. Plant organelle targeting cell penetrating peptides (POTCPPs) are peptides that form nanoparticles composed of short peptides and double-stranded DNA (dsDNA). The peptides have cell penetrating properties and specific organelle targeting properties. These properties enabled the peptides to translocate their cargo across the cell wall and outer plasma membrane of tissue cultured plant cells to deliver dsDNA to the mitochondria or the chloroplast and have it expressed in organello. The POTCPP transfection method represents the first report of a successful in vivo plant cell mitochondrial transfection and a peptide based plastid transfection within a monocot species. The POTCPP transfection method can be applied to organelle gene targeting, functional genomic and to the study of transient gene expression in tissue cultured plant cells.
Description
xii, 131 leaves : illustrations (chiefly coloured) ; 29 cm
Keywords
Mitochondria transfection , Plastid transfection , Cell penetrating peptide , Chloroplast targeting peptide , Plant cell , Plant mitochondria -- Research , Plant cell biotechnology -- Research , Plastids -- Research , Peptides -- Research , Gene targeting -- Research , Plant organelles -- Research , Plant cell membranes -- Research , Plant genetic engineering -- Research , Dissertations, Academic