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Control of substrate utilization by O-islands and S-loops in Escherichia coli O157:H7

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dc.contributor.advisor Thomas, James
dc.contributor.advisor Gannon, Victor
dc.contributor.author Paquette, Sarah-Jo
dc.date.accessioned 2012-07-20T00:25:32Z
dc.date.available 2012-07-20T00:25:32Z
dc.date.issued 2011
dc.identifier.uri http://hdl.handle.net/10133/3104
dc.description xx, 208 leaves : ill. (some col.) ; 29 cm en_US
dc.description.abstract Escherichia coli O157:H7 is an enteric pathogen that can cause severe gastrointestinal disease, sometimes leading to hospitalization and death. These bacteria have a variety of virulence factors that can be encoded for on pathogenicity islands (PAIs). The goal of this study was to characterize specific E. coli O157:H7 PAI deletion mutants using three methods: Phentotype Microarrays (PM), growth curves and survival curves were used to elucidate possible roles for the PAIs. Results from the PM study suggest that PAIs have a role in carbon substrate utilization; i.e., four of the O-island (OI) deletion mutants (OI-87, 98, 102 and 172) and an S-Loop (SL-72) deletion mutant exhibited differences in substrate utilization (gains and losses in utilization) compared to parental O157:H7 strains EDL933 (OI) and Sakai (SL), respectively. All of the mutants with the exception of the OI-135 mutant exhibited differences in level of substrate utilization for substrates shown to have important roles in the bacterium. Cell growth results showed that three OI deletion mutants (OI-55, 87 and 102) and the SL (SL-72) mutant exhibited a difference in rate of growth compared to the parental strains. Cell viability results showed that seven of the OI deletion mutants (OI-51, 55, 98, 108, 135, 172 and 176) exhibited different rates of decline in cell number when transferred to sterile water compared to the parental strain. The results show that removal of PAIs from E. coli O157:H7 can affect carbon utilization, growth and survival demonstrating the importance of PAIs in the ecology of these bacteria. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2011 en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Escherichia coli O157:H7 -- Research en_US
dc.subject Escherichia coli O157:H7 -- Genetics -- Experiments en_US
dc.subject Mutagenesis en_US
dc.subject DNA microarrays en_US
dc.subject Gene expression en_US
dc.subject Microbial mutation en_US
dc.subject Dissertations, Academic en_US
dc.title Control of substrate utilization by O-islands and S-loops in Escherichia coli O157:H7 en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US

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