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dc.contributor.supervisor Wieden-Kothe, Ute
dc.contributor.supervisor Roussel, Marc R.
dc.contributor.author Durand, Jessica
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2012-07-16T18:18:36Z
dc.date.available 2012-07-16T18:18:36Z
dc.date.issued 2010
dc.identifier.uri http://hdl.handle.net/10133/3101
dc.description vii, 83 leaves : ill. ; 29 cm en_US
dc.description.abstract Ribosome biogenesis is a crucial yet poorly understood and complex process in all cells. To date, most studies on eukaryotic ribosome biogenesis have relied on yeast genetics and whole cell analysis of ribosomal RNA processing. An early and critical step in ribosome biogenesis is the post-transcriptional modification of rRNA. Pseudouridylation is the most frequently occurring modification. Pseudouridylation is catalyzed by H/ACA small nucleolar ribonucleoproteins (snoRNPs) which are one of the two major classes of snoRNPs found within eukaryotes and archaea. H/ACA snoRNPs consist of four conserved core proteins Cbf5, Gar1, Nop10, and Nhp2 (eukaryotes), and a substrate specific H/ACA snoRNA. Mutations causing the rare inherited disease Dyskeratosis congenita are found in CBF5, NOP10, and NHP2. Here I report the purification of H/ACA protein Cbf5 in the presence of detergents. Additionally, I report initial in vitro RNA binding studies using Nhp2 and the snoRNA snR34 as well as the effects of Dyskeratosis congenita substitutions within Nhp2 on this interaction. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2010 en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Saccharomyces cerevisiae -- Research en_US
dc.subject Nucleoproteins -- Research en_US
dc.subject Post-translational modification -- Research en_US
dc.subject Ribosomes -- Research en_US
dc.subject Dissertations, Academic en_US
dc.title The biochemical characterization of Saccharomyces cerevisiae H/ACA small nucleolar ribonucleoproteins en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US
dc.degree.level Masters


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