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Characterization of the 16S/23S ribosomal RNA intergenic spacer regions of Listeria

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dc.contributor.advisor Thomas, James
dc.contributor.author Graham, Thomas A.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2007-04-02T01:08:37Z
dc.date.available 2007-04-02T01:08:37Z
dc.date.issued 1995
dc.identifier.uri http://hdl.handle.net/10133/26
dc.description xv, 131 leaves ; 29 cm. en
dc.description.abstract The 16S/23S ribosomal RNA (rRNA) intergenic space (IGS) regions from pathogenic and non-pathogenic species (spp.) of Listeria were characterized by the polymerase chain reaction (PCR) and DNA sequencing. DNA sequencing data for the small rRNA IGS region showed that this IGS was approximately 244 bp in length and was highly homologous (95 to 99 %) in five of the six Listeria spp examined; ie., L. monocytogenes, L. innocua, L. seeligeri, L. welshimeri, and L. ivanovii. A lower degree of homology (91 to 94 %) was detected in the large rRNA IGS region (ca. 494 bp) of these species. The DNA sequence data was used to develop two sets of oligonucleotide primers for PCR-based detection of the members of the genus Listeria. The first set of primers were Listeria genus-specific and, the second set of primers were L. monocytogenes-specific. en
dc.language.iso en_US en
dc.publisher Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1995 en
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts & Science) en
dc.subject Dissertations, Academic en
dc.subject Listeria monocytogenes en
dc.subject Listeriosis en
dc.subject Foodborne diseases en
dc.title Characterization of the 16S/23S ribosomal RNA intergenic spacer regions of Listeria en
dc.type Thesis en
dc.publisher.faculty Arts and Science
dc.publisher.department Department of Biological Sciences

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