Institutional Repository

Transgenic assays for the analysis of DNA repair in plants

DSpace/Manakin Repository

Show simple item record

dc.contributor.advisor Kovalchuk, Igor
dc.contributor.author Ilnytskyy, Yaroslav
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2007-05-17T14:49:25Z
dc.date.available 2007-05-17T14:49:25Z
dc.date.issued 2005
dc.identifier.uri http://hdl.handle.net/10133/263
dc.description xi, 132 leaves : ill. ; 29 cm. en
dc.description.abstract In this work we studied various aspects of DNA repair in plants, focusing mainly on point mutation repair and its interconnection with double-strand break repair. We were using transgenic point mutation and recombination substrates as a primary tool in our experiments. We have compared two transgenic homologous recombination assays (B-glucuronidase- and luciferase-based), analyzed the sensitivity of DNA repair machinery to ultraviolet radiation and assessed the involvement of AtKu80, Atm and AtXpd repair genes in point mutation repair. Ours study revealed the following: the luciferase-based recombination assay is more sensitive then B-glucuronidase-based; double-stand break repair machinery is sensitive to ultraviolet radiation, which results in increased pint mutation formation; chosen DNA repair genes might be impaired in point mutation repair, however further experimentations are needed to confirm this. en
dc.language.iso en_US en
dc.publisher Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2005 en
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en
dc.subject Dissertations, Academic en
dc.subject DNA repair en
dc.subject Mutation (Biology) en
dc.subject Transgenic plants en
dc.title Transgenic assays for the analysis of DNA repair in plants en
dc.type Thesis en
dc.publisher.faculty Arts and Science
dc.publisher.department Department of Biological Sciences

Files in this item

This item appears in the following Collection(s)

Show simple item record

Related Items

Search DSpace


Advanced Search

Browse

My Account

Statistics