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Radiation-induced deregulation of PiRNA pathway proteins : a possible molecular mechanism underlying transgenerational epigenomic instability

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dc.contributor.advisor Kovalchuk, Olga
dc.contributor.author Merrifield, Matthew
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2012-03-01T18:22:15Z
dc.date.available 2012-03-01T18:22:15Z
dc.date.issued 2011
dc.identifier.uri http://hdl.handle.net/10133/2617
dc.description ix, 123 leaves : ill. (some col.) ; 29 cm en_US
dc.description.abstract PiRNAs and their Piwi family protein partners are part of a germline specific epigenetic regulatory mechanism essential for proper spermatogenesis, silencing of transposable elements, and maintaining germline genome integrity, yet their role in the response of the male germline to genotoxic stress is unknown. Ionizing radiation (IR) is known to cause transgenerational genome instability that is linked to carcinogenesis. Although the molecular etiology of IR-induced transgenerational genomic instability is not fully understood, it is believed to be an epigenetically mediated phenomenon. IR-induced alterations in the expression pattern of key regulatory proteins involved in the piRNA pathway essential for paternal germline genome stability may be directly involved in producing epigenetic alterations that can impact future generations. Here we show whole body and localized X-irradiation leads to significant altered expression of proteins that are necessary for, and intimately involved in, the proper functioning of the germline specific piRNA pathway in mice and rats. In addition we found that IR-induced alterations to piRNA pathway protein levels were time and dose dependent. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Science, c2011 en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Piwi genes -- Effect of radiation on -- Research en_US
dc.subject Ionizing radiation -- Research en_US
dc.subject Genes -- Effect of radiation on -- Research en_US
dc.subject Germ cells -- Effect of radiation on -- Research en_US
dc.subject Genetic toxicology -- Research en_US
dc.subject Mice as laboratory animals en_US
dc.subject Rats as laboratory animals en_US
dc.subject Dissertations, Academic en_US
dc.title Radiation-induced deregulation of PiRNA pathway proteins : a possible molecular mechanism underlying transgenerational epigenomic instability en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US

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