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dc.contributor.supervisor Mosimann, Steven Janzen, Timothy William University of Lethbridge. Faculty of Arts and Science 2011-10-30T21:07:22Z 2011-10-30T21:07:22Z 2010
dc.description xi, 98 leaves : ill. (some col.) ; 29 cm en_US
dc.description.abstract Box C/D small ribonucleoproteins (box C/D sRNPs) are responsible for the 2’-O-methylation required for the complete maturation of precursor rRNA. Archaeal box C/D sRNPs, like eucarya, are composed of four components: a guide RNA (box C/D sRNA), an RNA binding protein (L7ae), a 2’-O-methyltransferase (Fibrillarin) and a structural protein (Nop5). Here we develop several approaches for studying box C/D sRNP assembly. In particular, we have used pulldown and mobility shift assays to identify box C/D sRNP assembly intermediates (Nop5-aFib and L7ae-sR1). We have also demonstrated that isothermal titration calorimetry (ITC) can be utilized to quantitatively characterize the energetics of formation for the L7ae-sRNA assembly intermediate. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2010 en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Nucleoproteins en_US
dc.subject RNA en_US
dc.subject Methylation en_US
dc.subject Archaebacteria -- Molecular aspects en_US
dc.subject Dissertations, Academic en_US
dc.title Subunit interactions within box C/D sRNPs en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry and Biochemistry en_US Masters

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