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Characterization of the genetic diversity and antimicrobial resistance in Mannheimia haemolytica from feedlot cattle

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dc.contributor.advisor Selinger, Brent
dc.contributor.author Klima, Cassidy L.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2011-08-19T19:22:00Z
dc.date.available 2011-08-19T19:22:00Z
dc.date.issued 2009
dc.identifier.uri http://hdl.handle.net/10133/2517
dc.description xi, 116 leaves : ill. ; 29 cm en_US
dc.description.abstract Mannheimia haemolytica is an opportunistic pathogen in cattle and the main bacterial agent in bovine respiratory disease. Despite its economic importance, few studies have characterized the genetic diversity of M. haemolytica, particularly from feedlots. Three genotyping techniques (BOX-PCR, (GTG)5-PCR and PFGE) were compared to discriminate M. haemolytica and strains from the family Pasteurellaceae. PFGE was the most discriminating and repeatable, although BOX-PCR was most accurate in clustering isolates together according to species. Mannheimia haemolytica was isolated from nasal swab samples collected from cattle upon entry and exit from two feedlots in southern Alberta. These were characterized by PFGE and antimicrobial susceptibility using a disk-diffusion assay. Select gene determinants were screened for using PCR. PFGE analysis revealed the isolates to be highly diverse. Ten percent of the isolates exhibited resistance. At present, the development and spread of antimicrobial resistance in M. haemolytica observed within the feedlots examined appears to be low. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2009 en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Bovine pneumonic pasteurellosis en_US
dc.subject Pathogenic bacteria en_US
dc.subject Cattle -- Pathogens -- Research en_US
dc.subject Cattle -- Diseases -- Research en_US
dc.subject Feedlots en_US
dc.subject Dissertations, Academic en_US
dc.title Characterization of the genetic diversity and antimicrobial resistance in Mannheimia haemolytica from feedlot cattle en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US

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