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Molecular methods and isolates of the entomopathogenic fungus Metarhizium anisolpliae for environmentally sustainable control of grasshoppers in Canada

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dc.contributor.advisor Johnson, Dan
dc.contributor.author Entz, Susan Carol
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2007-05-13T20:30:51Z
dc.date.available 2007-05-13T20:30:51Z
dc.date.issued 2005
dc.identifier.uri http://hdl.handle.net/10133/249
dc.description xv, 127 leaves : ill. (some col.) ; 28 cm. en
dc.description.abstract Metarhizium anisoplia var. acridum, a gyphomycetous fungus registered worldwide for grasshopper and locust control, is currently under consideration as a worldwide for grasshopper and locust control, is currently under consideration as a potential alternative to chemical insecticides for grasshopper control in Canada. Research in this thesis has contributed data required for the registration of biological control agents in Canada. A diagnostic PCR assay was developed for the specific detection of M. anisopliae var. acridum DNA. The assay was highly sensitive and effective for the detection of fungal DNA in infected grasshoppers. A survey of southern Alberta soils conducted in the spring of 2004 revealed the presence of Metarhizium spp. at low natural incidennce. Two indigenous isolates demonstrated pathogenicity when bioassayed against laboratory-reared and field collected grasshoppers. One of the isolates demonstrated virulence comparable to a commercial isolate. An analysis of historical weather data revealed that summer weather in the Prairie provinces should not preclude the efficacy of M. anisopliae var. acridum under local conditions. en
dc.language.iso en_US en
dc.publisher Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2005 en
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en
dc.subject Dissertations, Academic en
dc.subject Grasshoppers -- Biological control -- Canada en
dc.subject Biological insecticides en
dc.subject Entomophathogenic fungi en
dc.subject Grasshoppers -- Control -- Canada en
dc.title Molecular methods and isolates of the entomopathogenic fungus Metarhizium anisolpliae for environmentally sustainable control of grasshoppers in Canada en
dc.type Thesis en
dc.publisher.faculty Arts and Science
dc.publisher.department Department of Geography

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