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dc.contributor.supervisor Gannon, Victor
dc.contributor.supervisor Thomas, James E. Ross, Susan University of Lethbridge. Faculty of Arts and Science 2011-07-19T18:42:14Z 2011-07-19T18:42:14Z 2010
dc.description xii, 184 leaves : ill. ; 29 cm en_US
dc.description.abstract This thesis reports the development of Comparative Genomic Fingerprinting (CGF), a rapid genotyping method for Campylobacter jejuni that assesses the conservation status of 20 genes previously described as having high intraspecies variability based on comparative genomics studies. This novel method for genotyping C. jejuni, CGF was validated two-fold. First, by comparison to flaA restriction fragment length polymorphism analysis, and second a subset of isolates was validated using two higher resolution CGF assays assessing 35 and 119 genes. CGF was then tested in a molecular epidemiological study of C. jejuni isolated from environmental, animal and human clinical samples from southern Alberta. Reservoirs of infection, subtypes associated with higher incidence of human infection, and the persistence of prevalent subtypes in animal/environmental reservoirs were identified. This thesis demonstrates that CGF analysis is robust and can be used to rapidly assess genetic similarity of C. jejuni isolates and to detect epidemiologically relevant clonal groups. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2010 en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Campylobacter jejuni -- Research -- Alberta, Southern en_US
dc.subject Campylobacter infections -- Research -- Alberta, Southern en_US
dc.subject Molecular epidemiology en_US
dc.subject Dissertations, Academic en_US
dc.title Development of comparative genomic fingerprinting for molecular epidemiological studies of Campylobacter jejuni en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US Masters

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