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A molecular genetic approach to reducing the saturated fatty acid content of canola oil

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dc.contributor.advisor Weselake, Randall
dc.contributor.author Bondaruk, Mandy
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2007-04-25T19:37:12Z
dc.date.available 2007-04-25T19:37:12Z
dc.date.issued 2003
dc.identifier.uri http://hdl.handle.net/10133/145
dc.description xxi, 155 leaves : ill. ; 29 cm. en
dc.description.abstract Brassica napus is known to contain an endogenous and soluble stearoyl-acyl carrier protein (^918:0-ACP) desaturase, but does not express a palmitic (16:0)-ACP desaturase. Levels of 16:0 are low in canola oil and are associated with enhanced cholesterol biosynthesis in humans. In an attempt to further reduce the saturated fatty acid (SFA) content of canola oil, B. Napus L. cv Westar was transformed with a cDNA encoding a ^916:0-ACP desaturase from cat's claw (Doxantha unguis-cati L.). Arabidopsis thaliana was also transformed with this cDNA. Transformation of both oilseeds resulted in increased production of palmitoleic acid (^16:1) and many other effects of fatty acid composition. Overall, the SFA content did not decrease in either oilseed and investigation to why this effect occurred was examined using transgenic B. napus. Molecular genetic testing on second generation B. napus also determined the plants contained the cDNA of interest and were transcribing the cDNA. en
dc.language.iso en_US en
dc.publisher Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2003 en
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en
dc.subject Saturated fatty acids en
dc.subject Canola oil en
dc.subject Fatty acids -- Synthesis en
dc.subject Oilseeds en
dc.subject Oilseed plants -- Composition en
dc.subject Molecular genetics en
dc.subject Dissertations, Academic en
dc.title A molecular genetic approach to reducing the saturated fatty acid content of canola oil en
dc.type Thesis en
dc.publisher.faculty Arts and Science
dc.publisher.department Department of Chemistry and Biochemistry

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