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Stimulation of microsomal diacylglycerol acyltransferase activity from microspore-derived cell suspension cultures of oilseed rape

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dc.contributor.advisor Weselake, Randall
dc.contributor.author Byers, Susan D.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2007-04-24T19:51:20Z
dc.date.available 2007-04-24T19:51:20Z
dc.date.issued 1999
dc.identifier.uri http://hdl.handle.net/10133/101
dc.description xiii, 95 leaves : ill. ; 28 cm. en
dc.description.abstract Several factors including an unidentified endogenous substance were found to stimulate microsomal diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) from a microspore-derived cell suspension culture of oilseed rape (Brassica napus L. cv Jet Neuf). Mg2+ salts were found to stimulate microsomal DGAT 14 to 23-fold. ATP and CoA were also found to stimulate the enzyme 2.4 and 12 fold respectively, although the effects were decreased in the presence of high Mg2+ concentrations. While microsomal DGAT activity was only slightly increased by the concentration of exogenous diacylglycerol in the reaction mixture it was increased substantially by the addition of exogenous phosphatidate. Other phospholipids tested were not found to have this stimulatory effect. During attempts to investigate possible covalent modification of the enzyme, the soluble fraction obtained from cell suspension homogenate was found to contain a small metastable organic molecule(s) which stimulated DGAT activity. Stimulation of microsomal DGAT by this factor was concentration dependent but not dependent on preincubation time. en
dc.language.iso en_US en
dc.publisher Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1999 en
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en
dc.subject Oilseed plants en
dc.subject Fatty acids -- Synthesis en
dc.subject Enzymes en
dc.subject Canola oil en
dc.subject Oilseeds en
dc.subject Acyltransferases en
dc.subject Phosphorylation en
dc.subject Dissertations, Academic en
dc.title Stimulation of microsomal diacylglycerol acyltransferase activity from microspore-derived cell suspension cultures of oilseed rape en
dc.type Thesis en
dc.publisher.faculty Arts and Science
dc.publisher.department Department of Chemistry and Biochemistry

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