SynBridge Infrastructure

SynBridge is equipped for a variety of synthetic biology, biochemistry and molecular biology applications, including cell culture, PCR and electrophoresis, detection and visaluzation, and transfection.

Our most popular item is our fluorescence activated cell sorter (FACS), the BD FACSAria™ Fusion Cell Sorter.

FACS

Below is a list of equipment available in SynBridge.  SynBridge equipment can be booked, and for further information please contact the SynBridge Director, Dr. Laura Keffer-Wilkes (synbridge@uleth.ca).  SynBridge is located in Science Commons SA9162.


General equipment

Centrifuges
- Avanti™ JXN-26 high-speed centrifuge
- Heraeus™ Multifuge x3R
- Thermo Scientific™ Sorvall™ Legend™ Micro17 microcentrifuge
Fridges and freezers
- 4°C, -20°C and -80°C
Heating equipment
- Block heater and water bath
Analytical balance
pH meter
Vortex mixer
Safety equipment
- Fume hood
- Biological safety cabinet (Class II, type A2)


Cell Culture

Incubators
- VWR® Gravity Convection Incubator
- Thermo Scientific™ Forma™ 310 Direct-Heat CO2 Incubator
Shakers
- Eppendorf Innova® 42R shaker
- MBI Orbital Shaker
Cell counters and sorters
- BD FACSAria™ Fusion Cell Sorter
- Luna-II™ Automated cell counter
- Moxi™ Z Mini Automated Cell Counter
VWR® inverted microscope
- Biological safety cabinet (Class II, type A2)

Protein Purification

ÄKTA Pure 25 L Chromatography System


PCR

Eppendorf™ Mastercycler™ Nexus Gradient Thermal Cycler
Eppendorf™ Mastercycler™ Nexus Gradient eco
StepOnePlus™ Real-Time PCR System


Electrophoresis

FisherBiotech™ Horizontal Electrophoresis Systems
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell


Detection

GE Amersham™ Imager 600 RGB (for gels and membranes)
Micro-volume Spectrophotometer: BioDrop µLITES
Spectrophotometer: Biochrom WPA Biowave II UV/Vis 5nm bandpass
Microplate reader: SpectraMax® i3x Multi-Mode Detection Platform


Transfection

Gene Pulser Xcell™ Electroporation System Flier